Determination of DON and Deepoxy-DON in liquid manure of biogas production

Publication Type

Contribution to conference

Authors

Schollenberger, M., Rüfle, M., Suchy, S., Dejanovic, C., Frauz, B., Oechsner, H. and Drochner, W.

Year of publication

2006

Published in

28. Mykotoxin Workshop

Editor

Society for Mycotoxin Research

Series/labeling

Mykotoxin Workshop

Page (from - to)

88

Conference location

Bydgoszcz (Polen)

Conference date

29.-31.05.2006

Keywords

biogas production, Deepoxy-Don, DON, liquid manure

Every year batches of wheat are harvested which are not suitable for consumption because of high contamination with Fusarium and Fusarium toxins, particularly with the trichothecene deoxynivalenol (DON). Therefore methods of safe and economically effective disposal of these grain charges are urgently needed. In a research approach at the Univ. of Hohenheim under financial support of the German FNR (Fachagentur für Nachwachsende Rohstoffe) the prerequisites of a potential deactivation of Fusarium spores as well as of a degradation of DON during biogas production are evaluated. A precondition of this study was the capability of a selective and sensitive determination of DON and its degradation product, Deepoxy-DON, in liquid manure of biogas production, Due to the aqueous matrix of the liquid manure, a sample preparation using liquid/liquid partitioning on kieselguhr material was developed. Measurement of toxins was done with two different methods. First DON and Deepoxy-DON were determined by GC/MS after derivatisation with trifluoraceticanhydride (Schollenberger et al., 2005). Derivatisation procedure and measurement conditions were modified to allow for selective and sensitive analysis of these two substances as well as of a spectrum of further trichothecenes of the A- as well as of the B-type out of liquid manure. This could be of interest when Substrates other than wheat are used in biogas production. Furthermore a rapid method for the determination of DON with HPLC as described by Sano et al (1987) and Lepschy von Gleissenthall (2000) was adapted to fit for analysis of DON and DeepoxyDON out of liquid manure of biogas production. The toxins were separated by reversed-phase chromatography and measured using fluorescence detection after post-column derivatisation with NaOH and methylacetoacetate. Correlation between both methods was satisfactory.