Distribution of a spectrum of 16 Fusarium toxins during dry milling of corn
- Publication Type
- Contribution to conference
- Authors
- Schollenberger, M., H.-M. Müller, W. Drochner
- Year of publication
- 2007
- Published in
- 29. Mycotoxin-Workshop
- Editor
- Ges. für Mykotoxinforschung e.V.
- Page (from - to)
- 108
- Conference name
- Mycotoxin-Workshop
- Conference location
- Fellbach
- Conference date
- 14.-16.05.2007
- Keywords
- corn, Fusarium toxins
Corn plants are susceptible to Fusarium infestation and a spectrum of Fusarium toxins has been described in the literature for corn kernels. The redistribution of nivalenol (NIV), deoxynivalenol (DON), T-2 Toxin (T-2) and in addition of zearalenone (ZEA) was investigated for wet milling that of ZEA for dry milling of corn. There is a lack of Information about the face of trichothecenes for dry milling process of corn.In the present study the redistribution of 13 trichothecenes of the A and of the B-type as well as of ZEA, ?- and ?-zearalenol (?-ZOL and ?-ZOL) during dry milling of corn was investigated. Raw corn and outcoming fractions belonging to two different batches were obtained from a corn processing factory. The samples were analysed for the B-type trichothecene toxins NIV, fusarenon-X (FUS-X), DON, 3 and 15- acetyldeoxynivalenol (3- and 15-ADON) as well as the A-type trichothecenes T-2, HT-2 toxin (HT-2), T-2 triol, T-2 tetraol, neosolaniol (NEO), 4,15-diacetoxyscirpenol (4,15-DAS), 15-monoacetoxyscirpenol (15MAS) and scirpentriol (SCIRP) using GC/MS and for ZEA, ?-ZOL and ?-ZOL using HPLC/fluorescence detection. Detection limits ranged between 1 and 15 µg/kg out of oil samples and between 1 and 19 µg/kg for all other fractions of dry milling process.In samples of raw corn from both batches 7 and 4 trichothecenes, respectively, as well as ZEA were detected. Further toxins were found in fractions of the dry milling process. In general toxins were accumulated in germ, bran and expeller and reduced in the fractions grit and flour, compared to the original substance. Concerning the redistribution between expeller and crude oil the polar alcohols NIV, DON, T-2 tetraol and SCIRP were exclusively located in the expeller, no toxin was found in crude oil fraction. Out of the partially acetylated toxins, FUS-X, 3-ADON and MAS, contained in the germ with concentrations below 100 µg/kg were found in expeller but not in oil. 15-ADON .....the more polar toxins.