Simultaneous determination of a spectrum of trichothecene toxins out of residuals of biogas production

Publication Type

Journal contribution

Authors

Schollenberger M., Müller H.-M., Rüfle M., Suchy S., Deianovic C., Frauz B., Oechsner H. and Drochner W.

Year of publication

2008

Published in

Journal of Chromatography A

Pubisher

Elsevier B.V.

Band/Volume

1193/1-2

ISBN / ISSN / eISSN

0021-9673

DOI

10.1016/j.chroma.2008.04.002

Page (from - to)

92-96

Keywords

Biogasproduktion, Deepoxy-Don, Gaschromatographie, HPLC-method, Trichothecenes

A sensitive and selective method for the simultaneous determination of a spectrum of trichothecenes in residuals of biogas production has been developed. It comprises Sample clean-up by liquid/liquid partition for digested manure and solid phase extraction for digested solid phase. Quantification of A- and B-type trichothecenes as their trifluoroacetyl derivatives is performed by gas chromatography mass spectrometry (GC/MS), that of B-type trichothecenes alternatively by high-performance liquid chromatography (HPLC). Fluorescence detection (FLD) after post-column derivatisation using methylacetoacetate and ammonium acetate after alkaline decomposition of toxins was applied. Detection limits in digestates were between 1 and 30µg/l and 20 and 50µg/l for GC/MS and HPLC/FLD, respectively. Recovery rates were between 52 and 129% for GC/MS detection with the exception of T-2 tetraol with 22%, and between 56 and 123% for HPLC/FLD