Comparison of caecal chyme and faeces of pigs as inoculum for the fermentation of hay, oat bran and gelatine with the modified Hohenheim gas test

Publikations-Art

Kongressbeitrag

Autoren

Zacharias, B., Duda, D., Buske, B., Tafaj, M., Piepho, H.-P., Drochner, W.

Erscheinungsjahr

2004

Veröffentlicht in

Proceedings of the Society of Nutrition Physiology

Herausgeber

Gesellschaft für Ernährungsphysiologie

Verlag

DLG-Verlags-GmbH , Frankfurt

Serie/Bezeichnung

Proc. Soc. Nutr. Physiol.

Seite (von - bis)

90

Tagungsname

58. Tagung

Tagungsort

Göttingen

Tagungsdatum

09.-11.03.2004

Schlagworte

Caecum, chyme, Hohenheim gas test, Inoculum, pig

Faecal samples are often used as inoculum for the simulation of fermentation processes in the large intestine of the pig in vitro. Faecal sampling is less labour intensive and the disturbance to the animal is minimized as compared to the sampling of chyme. The use of faeces as inoculum requires that the microbial population in the faeces is comparable to the microbial population in the large intestine. The aim of the present study was to evaluate wether faeces can be used instead of caecal chyme for the inoculation of different feedstuffs in vitro. The gas production and the formation of total volatile fatty acids of hay, oat bran and gelatine were determined with faeces and chyme as inocula in an in vitro system in which fermentations were conducted in 100 ml gas-tight glass syringe barrels. An amount of buffering substances was added to the system which was comparable to the amount of buffering substances in the chyme of pigs. Chyme was collected from 6 pigs fitted with T-cannulas at the caecum. Faeces were collected from the rectum of the animals. 100 mg of the samples were incubated at 39 °C for 12, 24 and 48 h. In all samples the gas production and the concentration of volatile fatty acids showed lower values within 12 h when faeces was used as inoculum as compared to chyme. Only for hay the gas production and the amount of volatile fatty acids increased further within 24 h to 48 h with both inocula. No differences in the production of gas and in the amount of volatile fatty acids between the two inocula were obtained after 24 h and 48 h. The fermentation starts more rapid when chyme is used as inoculum. After 24 h in oat bran and gelatine the fermentation is finished in both inocula. The further fermentation of hay with both inocula might be caused by the higher amount of cellulose in this sample. As there were no significant differences between the two inocula after 24 h and 48 h, faeces can be used instead of chyme as inoculum for the simulation of ...