Degradation of Deoxynivalenol during anaerobic fermentation
- Publikations-Art
- Kongressbeitrag
- Autoren
- Frauz, B., H. Oechsner, M. Schollenberger, W. Drochner
- Erscheinungsjahr
- 2007
- Veröffentlicht in
- 29. Mycotoxin-Workshop
- Herausgeber
- Ges. für Mykotoxinforschung e.V.
- Seite (von - bis)
- 87
- Tagungsname
- Mycotoxin-Workshop
- Tagungsort
- Fellbach
- Tagungsdatum
- 14.-16.05.2007
- Schlagworte
- anaerobic fermentation, Deoxynivalenol
A current research project at the State Institute of Farm machinery and Farm structures and the Institute of Animal Nutrition at the University of Hohenheim was launched to clarify the terms and conditions for a potential intermixture of Fusarium-contaminated substrate in agricultural biogas plants. The design of this research approach was set to modify fermentation in order to gain the aforementioned deactivation of the anti nutritives, such as Deoxynivalenol. One option for evaluation of biogas potential is to determine appearing CH4 yields of standard inoculated manure combined with the toxic substrate using a batch fermentation test, the so called Hohenheim biogas yield test [1] after VDI 4630 (directive for fermentation) [4]. The small dimension of the experimental facility, consisting of about 130 flask samplers with a volume of 100 ml allows an accomplishment out of a large number of repetitions or parallel tests with a reduced spare requirement. Other than the mentioned methane production also the fate of Fusarium was recorded [3]. To evaluate the microbiological transactions deoxynivalenol standard solution in standard liquid manure und DON-free wheat flour were applied into the test system and incubated at 35°C. Samples were taken at specific Bates and analysed for the content of DON and its metabolite Deepoxydeoxynivalenol by HPLC/FLD or GC/MS after liquid/liquid extraction using kieselguhr material [2]. A complete degradation of DON was observed by a coevally detection of Deepoxynivalenol