In-vitro studies on the capacity of some macromolecules to adsorb deoxynivalenol and zearalenone

Publikations-Art

Kongressbeitrag

Autoren

Schollenberger M., Müller H.-M., Terry-Jara H. and Drochner W.

Erscheinungsjahr

2008

Veröffentlicht in

Conference Abstracts

Herausgeber

Gesellschaft für Mykotoxinforschung e.V.

Serie/Bezeichnung

Mycotoxin-Workshop

Seite (von - bis)

134

Tagungsort

Utrecht (Niederlande)

Tagungsdatum

28.04.2008

Schlagworte

Deoxynivalenol, mycotoxins, Zearalenone

Introduction: Mycotoxin-binding agents are added to feedstuffs in order to adsorb mycotoxins in the gastrointestinal tract, thus preventing the uptake in the blood and subsequent distribution to target organs. An in vitro system was developed to study the efficacy of different macromolecules as adsorbing substances to detoxify deoxynivalenol (DON), zearalenone (ZEA) as well as alpha- and beta-zearalenole (alpha- and beta-ZOL) in situ. As pigs are very susceptible to Fusarium toxin contamination of feedstuffs the conditions of porcine gastrointestinal tract were simulated. The following macromolecules were tested: cellulose, lignocellulose, fiber of oats, pectin, tannin.

Materials and Methods: Artificial gastric and intestinal fluid containing pepsin and pancreatin, respectively, were prepared according to United States Pharmacopeia (Joel et al, 1995) and were spiked with DON, ZEA, alpha- and beta-ZOL stock solutions to correspond with a meal containing 10 mg DON and 1 mg ZEA per kg of diet, concentration of macromolecule corresponded to a content of 3 % in feed at a stomach dry matter content of 25 % as reported by Döll et al. (2004). Gastric adsorption and intestinal desorption as well as intestinal adsorption were tested by incubation at 37 °C for four hours respectively. After incubation sample clean-up was by using liquid/liquid partition on diatomaceous earth, measurement of toxins was by HPLC.

Results: Lignocellulose was the only substance tested which showed relevant ability in binding ZEA and its derivatives. None of the materials tested was able to bind DON in noteable amounts under the conditions applied.

Discussion and Conclusion: As digestive enzymes among others may interfere with toxin adsorption in the gastrointestinal tract of animals (Döll et al. 2004), in the in vitro model presented not only pH, temperature and transit time but also enzymatic influences have been considered. Thus pepsin and pancreatin isolated from porcine tissues were incorporated into the media. The in-vitro system deployed allows for screening substances for their ability to adsorb mycotoxins. Of the macromolecules tested lignocellulose was able to adsorb ZEA and its derivatives in relevant amounts.